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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FANCG Polyclonal antibody

FANCG Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 10215-1-AP

Synonyms

XRCC9, Protein FACG, Fanconi anemia group G protein, FAG, DNA repair protein XRCC9

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using FANCG Polyclonal antibody (10215-1-AP)

    WB analysis of Jurkat using 10215-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using FANCG Polyclonal antibody (10215-1-AP)

    WB analysis of mouse thymus using 10215-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using FANCG Polyclonal antibody (10215-1-AP)

    WB analysis of mouse testis using 10215-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FANCG Polyclonal antibody (10215-1-AP)

    WB analysis of K-562 using 10215-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FANCG Polyclonal antibody (10215-1-AP)

    WB analysis of HeLa using 10215-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using FANCG Polyclonal antibody (10215-1-AP)

    IP experiment of mouse testis using 10215-1-AP

    IP result of anti-FANCG (IP:10215-1-AP, 4ug; Detection:10215-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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Tested Applications

Positive WB detected inJurkat cells, mouse testis tissue, K-562 cells, HeLa cells, mouse thymus tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10215-1-AP targets FANCG in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FANCG fusion protein Ag0249 相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group G
Calculated molecular weight 69 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC000032
Gene symbol FANCG
Gene ID (NCBI) 2189
RRIDAB_2231541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FANCG, also named as XRCC9, is a vDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's a candidate tumor suppressor gene. FANCA and FANCG are critical for the localization of the FANC core complex. FANCA and FANCG form a stable complex with a molecular weight of ∼500 kDa that is predicted to be a heterotetramer (PMID: 32002546).

Protocols

Product Specific Protocols
WB protocol for FANCG antibody 10215-1-APDownload protocol
IP protocol for FANCG antibody 10215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

Authors - Dinh Duc Nguyen
View Article
humanIF

J Cancer

Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma.

Authors - Yi Liu
View Article
humanWB

Clin Transl Oncol

Therapeutic benefits of niraparib tosylate as radio sensitizer in esophageal squamous cell carcinoma: an in vivo and in vitro preclinical study.

Authors - Yuzhong Cui
View Article