FANCI Monoclonal antibody

FANCI Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10E6

Cat no : 67304-1-Ig

Synonyms

Protein FACI, Fanconi anemia group I protein


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Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, LNCaP cells, A549 cells, Raji cells, Daudi cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67304-1-Ig targets FANCI in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FANCI fusion protein Ag15153 相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group I
Calculated molecular weight 1328 aa, 149 kDa
Observed molecular weight 149 kDa, 142 kDa
GenBank accession numberBC004277
Gene symbol FANCI
Gene ID (NCBI) 55215
RRIDAB_2882566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FANCI antibody 67304-1-IgDownload protocol
IHC protocol for FANCI antibody 67304-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway

Authors - Zixian Huang
humanWB,IHC

Front Immunol

FANCI serve as a prognostic biomarker correlated with immune infiltrates in skin cutaneous melanoma

Authors - Zhenguo Cai
humanWB

Adv Sci (Weinh)

The Compromised Fanconi Anemia Pathway in Prelamin A-Expressing Cells Contributes to Replication Stress-Induced Genomic Instability

Authors - Pengqing Nie