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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FANCL Polyclonal antibody

FANCL Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19187-1-AP

Synonyms

PHF9, Fanconi anemia-associated polypeptide of 43 kDa, Fanconi anemia group L protein, FAAP43, EC:2.3.2.27

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using FANCL Polyclonal antibody (19187-1-AP)

    WB analysis of human brain using 19187-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using FANCL Polyclonal antibody (19187-1-AP)

    WB analysis of human brain using 19187-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FANCL Polyclonal antibody (19187-1-AP)

    WB analysis of HeLa using 19187-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FANCL Polyclonal antibody (19187-1-AP)

    WB analysis of HEK-293 using 19187-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19187-1-AP (FANCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FANCL Polyclonal antibody (19187-1-AP)

    IF Staining of HeLa using 19187-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 19187-1-AP (FANCL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, HeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19187-1-AP targets FANCL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FANCL fusion protein Ag6192 相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group L
Calculated molecular weight 43 kDa
Observed molecular weight 41-43 kDa
GenBank accession numberBC054517
Gene symbol FANCL
Gene ID (NCBI) 55120
RRIDAB_10597246
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fanconi anemia (FA) is a hereditary bone marrow failure syndrome associated with developmental defects and cancer predisposition. The FA nuclear core complex consists of FANCA, B, C, E, F, G, L, and M. FANCL is a RING-type E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI, a key functional role facilitated by other members of the nuclear core complex (PMID: 23783032). FANCL plays an important role in the repair of cisplatin-induced DNA damage (PMID: 26385482) and participates directly in support of stem cell function (PMID: 23783032). FANCL can be detected as 40-43 kDa (full-length) and ~28 kDa.

Protocols

Product Specific Protocols
WB protocol for FANCL antibody 19187-1-APDownload protocol
IF protocol for FANCL antibody 19187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols