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  • Featured Product
  • KD/KO Validated

FANCL Monoclonal antibody, PBS Only

FANCL Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E2B6

Cat no : 66639-1-PBS

Synonyms

FAAP43, FANCL, Fanconi anemia group L protein, PHF9, POG

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FANCL Monoclonal antibody (66639-1-Ig)

    WB analysis of HeLa using 66639-1-Ig (same clone as 66639-1-PBS)

    HeLa cells and HEK-293 cells were subjected to SDS PAGE followed by western blot with 66639-1-Ig (FANCL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66639-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FANCL Monoclonal antibody (66639-1-Ig)

    WB analysis of HEK-293 using 66639-1-Ig (same clone as 66639-1-PBS)

    WB result of FANCL antibody (66639-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FANCL transfected HEK-293 cells. This data was developed using the same antibody clone with 66639-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FANCL Monoclonal antibody (66639-1-Ig)

    IF Staining of HeLa using 66639-1-Ig (same clone as 66639-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCL antibody (66639-1-Ig, Clone: 6E2B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66639-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66639-1-PBS targets FANCL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FANCL fusion protein Ag6192 相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group L
Calculated molecular weight 43 kDa
Observed molecular weight 41-43 kDa
GenBank accession numberBC054517
Gene symbol FANCL
Gene ID (NCBI) 55120
RRIDAB_2881998
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fanconi anemia (FA) is a hereditary bone marrow failure syndrome associated with developmental defects and cancer predisposition. The FA nuclear core complex consists of FANCA, B, C, E, F, G, L, and M. FANCL is a RING-type E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI, a key functional role facilitated by other members of the nuclear core complex (PMID: 23783032). FANCL plays an important role in the repair of cisplatin-induced DNA damage (PMID: 26385482) and participates directly in support of stem cell function (PMID: 23783032). FANCL can be detected as 40-43 kDa (full-length) and ~28 kDa.