Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
12954-1-AP targets FANCM in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FANCM fusion protein Ag3618 相同性解析による交差性が予測される生物種 |
| Full Name | Fanconi anemia, complementation group M |
| Calculated molecular weight | 2048 aa, 232 kDa |
| Observed molecular weight | 250 kDa, 232 kDa |
| GenBank accession number | BC036056 |
| Gene Symbol | FANCM |
| Gene ID (NCBI) | 57697 |
| RRID | AB_10644146 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYD8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FANCM (Fanconi anemia group M protein) is a DNA-dependent ATPase component of the Fanconi anemia (FA) core complex (PMID: 16116422). It is required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage (PMID:16116422; 19423727; 20347428). FANCM is localized in the cell nucleus and can bind double-stranded DNA (dsDNA), fork-structured DNA (fsDNA), and Holliday junction substrates (PMID: 20347428; 20347429).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FANCM antibody 12954-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J m6 A deposition is regulated by PRMT1-mediated arginine methylation of METTL14 in its disordered C-terminal region. | ||
Onco Targets Ther The Regulatory Mechanism and Biological Significance of Mitochondrial Calcium Uniporter in the Migration, Invasion, Angiogenesis and Growth of Gastric Cancer. | ||
mSystems Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host-pathogen interaction | ||
Cell Prolif Lin28a forms an RNA-binding complex with Igf2bp3 to regulate m6A-modified stress response genes in stress granules of muscle stem cells |