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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HepG2 cells using FANCM Recombinant antibody (82897-2-RR)

    IP experiment of HepG2 using 82897-2-RR (same clone as 82897-2-PBS)

    IP result of anti-FANCM (IP:82897-2-RR, 4ug; Detection:82897-2-RR 1:500) with HepG2 cells lysate 400 ug. This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FANCM Recombinant antibody (82897-2-RR)

    IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FANCM Recombinant antibody (82897-2-RR)

    IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FANCM Recombinant antibody (82897-2-RR)

    IF Staining of HeLa using 82897-2-RR (same clone as 82897-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCM antibody (82897-2-RR, Clone: 230233G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using FANCM Recombinant antibody (82897-2-RR)

    FC experiment of PC-3 using 82897-2-RR (same clone as 82897-2-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug FANCM Recombinant antibody (82897-2-RR, Clone:230233G5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

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FANCM Recombinant antibody, PBS Only

FANCM Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 82897-2-PBS
Clone No.230233G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82897-2-PBS targets FANCM in IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FANCM fusion protein Ag34447 相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group M
GenBank accession numberBC140776
Gene Symbol FANCM
Gene ID (NCBI) 57697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IYD8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.