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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAP Recombinant antibody

FAP Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

241129B4

Cat no : 84018-4-RR

Synonyms

FAPA, EC:3.4.21.-, EC:3.4.14.5, Dipeptidyl peptidase FAP, Antiplasmin-cleaving enzyme FAP, soluble form

Validation Data Gallery

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  • Western Blot (WB) analysis of U-87 MG cells using FAP Recombinant antibody (84018-4-RR)

    WB analysis of U-87 MG using 84018-4-RR

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 84018-4-RR (FAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using FAP Recombinant antibody (84018-4-RR)

    FC experiment of Transfected HEK-293T using 84018-4-RR

    1x10^6 HEK-293T cells or Transfected HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone:241129B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 84018-4-RR

    Affinity and Kinetic Characterization of 84018-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84018-4-RR against Human FAP were performed. The affinity constant is 0.126 nM.

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Tested Applications

Positive WB detected inU-87 MG cells
Positive FC detected inTransfected HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84018-4-RR is FAP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name fibroblast activation protein, alpha
Calculated molecular weight88 kDa
Observed molecular weight 80 kDa
GenBank accession numberNM_004460.4
Gene symbol FAP
Gene ID (NCBI) 2191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).

Protocols

Product Specific Protocols
WB protocol for FAP antibody 84018-4-RRDownload protocol
FC protocol for FAP antibody 84018-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols