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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAR2 Polyclonal antibody

FAR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27717-1-AP

Synonyms

SDR10E2, MLSTD1, HEL-S-81

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAR2 Polyclonal antibody (27717-1-AP)

    WB analysis using 27717-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27717-1-AP (FAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using FAR2 Polyclonal antibody (27717-1-AP)

    IHC staining of human colon using 27717-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27717-1-AP (FAR2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using FAR2 Polyclonal antibody (27717-1-AP)

    IHC staining of human colon using 27717-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27717-1-AP (FAR2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27717-1-AP targets FAR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAR2 fusion protein Ag26819 相同性解析による交差性が予測される生物種
Full Name fatty acyl CoA reductase 2
Calculated molecular weight 515 aa, 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC022267
Gene symbol FAR2
Gene ID (NCBI) 55711
RRIDAB_3669618
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fatty acyl-CoA reductase 2 (FAR2), also known as MLSTD1, belongs to the fatty acyl-CoA reductase family. FAR2 catalyzes the reduction of saturated but not unsaturated C16 or C18 fatty acyl-CoA to fatty alcohols (PMID: 24009241). FAR2 may play a role in the production of ether lipids/plasmalogens and wax monoesters which synthesis requires fatty alcohols as substrates. FAR2 has 2 isoforms with the molecular mass of 49 kDa and 59 kDa (Refer to Uniprot).

Protocols

Product Specific Protocols
WB protocol for FAR2 antibody 27717-1-APDownload protocol
IHC protocol for FAR2 antibody 27717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols