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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAR2 Polyclonal antibody

FAR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27717-1-AP

Synonyms

SDR10E2, MLSTD1, HEL-S-81

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAR2 Polyclonal antibody (27717-1-AP)

    WB analysis using 27717-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27717-1-AP (FAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using FAR2 Polyclonal antibody (27717-1-AP)

    IHC staining of human colon using 27717-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27717-1-AP (FAR2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using FAR2 Polyclonal antibody (27717-1-AP)

    IHC staining of human colon using 27717-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27717-1-AP (FAR2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27717-1-AP targets FAR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAR2 fusion protein Ag26819 相同性解析による交差性が予測される生物種
Full Name fatty acyl CoA reductase 2
Calculated molecular weight 515 aa, 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC022267
Gene symbol FAR2
Gene ID (NCBI) 55711
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fatty acyl-CoA reductase 2 (FAR2), also known as MLSTD1, belongs to the fatty acyl-CoA reductase family. FAR2 catalyzes the reduction of saturated but not unsaturated C16 or C18 fatty acyl-CoA to fatty alcohols (PMID: 24009241). FAR2 may play a role in the production of ether lipids/plasmalogens and wax monoesters which synthesis requires fatty alcohols as substrates. FAR2 has 2 isoforms with the molecular mass of 49 kDa and 59 kDa (Refer to Uniprot).

Protocols

Product Specific Protocols
WB protocol for FAR2 antibody 27717-1-APDownload protocol
IHC protocol for FAR2 antibody 27717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols