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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FARP1 Polyclonal antibody (24014-1-AP)

    WB analysis using 24014-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24014-1-AP (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FARP1 Polyclonal antibody (24014-1-AP)

    WB analysis using 24014-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24014-1-AP (FARP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using FARP1 Polyclonal antibody (24014-1-AP)

    IP experiment of HeLa using 24014-1-AP

    IP result of anti-FARP1 (IP:24014-1-AP, 4ug; Detection:24014-1-AP 1:1000) with HeLa cells lysate 1200 ug.

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FARP1 Polyclonal antibody

FARP1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 24014-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Pleckstrin homology domain-containing family C member 2, PH domain-containing family C member 2, FERM, RhoGEF and pleckstrin domain-containing protein 1, FERM, ARHGEF and pleckstrin domain-containing protein 1, Chondrocyte-derived ezrin-like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HEK-293 cells, HeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24014-1-AP targets FARP1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FARP1 fusion protein Ag21225 相同性解析による交差性が予測される生物種
Full Name FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
Calculated molecular weight 1076 aa, 122 kDa
Observed molecular weight122 kDa
GenBank accession numberBC071592
Gene Symbol FARP1
Gene ID (NCBI) 10160
RRIDAB_3085720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9Y4F1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FARP1 is a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth.

Protocols

Product Specific Protocols
WB protocol for FARP1 antibody 24014-1-APDownload protocol
IP protocol for FARP1 antibody 24014-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols