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FARSA Polyclonal antibody
FARSA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 18121-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, K-562 cells, MCF-7 cells, HepG2 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells, HeLa cells, A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:4000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
ELISA | See 1 publications below |
RIP | See 1 publications below |
Product Information
18121-1-AP targets FARSA in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FARSA fusion protein Ag12647 相同性解析による交差性が予測される生物種 |
Full Name | phenylalanyl-tRNA synthetase, alpha subunit |
Calculated molecular weight | 58 kDa |
Observed molecular weight | 57 kDa |
GenBank accession number | BC006495 |
Gene symbol | FARSA |
Gene ID (NCBI) | 2193 |
RRID | AB_2102509 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for FARSA antibody 18121-1-AP | Download protocol |
IHC protocol for FARSA antibody 18121-1-AP | Download protocol |
IF protocol for FARSA antibody 18121-1-AP | Download protocol |
IP protocol for FARSA antibody 18121-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. | ||
Clin Genet FARS1-related disorders caused by bi-allelic mutations in cytosolic phenylalanyl-tRNA synthetase genes: Look beyond the lungs! | ||
Nat Commun Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.
| ||
Autoimmun Rev Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase | ||
Nat Commun Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA |