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  • KD/KO Validated

FARSA Polyclonal antibody

FARSA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 18121-1-AP

Synonyms

Phenylalanine--tRNA ligase alpha subunit, FARSLA, FARSL, FARS, EC:6.1.1.20


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Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, HepG2 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18121-1-AP targets FARSA in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FARSA fusion protein Ag12647 相同性解析による交差性が予測される生物種
Full Name phenylalanyl-tRNA synthetase, alpha subunit
Calculated molecular weight 58 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC006495
Gene symbol FARSA
Gene ID (NCBI) 2193
RRIDAB_2102509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FARSA antibody 18121-1-APDownload protocol
IHC protocol for FARSA antibody 18121-1-APDownload protocol
IF protocol for FARSA antibody 18121-1-APDownload protocol
IP protocol for FARSA antibody 18121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
humanWB

Clin Genet

FARS1-related disorders caused by bi-allelic mutations in cytosolic phenylalanyl-tRNA synthetase genes: Look beyond the lungs!

Authors - Luise A Schuch
human,rat,mouseIF,ELISA

Nat Commun

Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.

Authors - Qian Zhou
  • KO Validated
humanWB,IP

Autoimmun Rev

Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase

Authors - Yoshinao Muro
human,mouseWB,IF,RIP

Nat Commun

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Authors - Mirjana Malnar Črnigoj