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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FARSA Monoclonal antibody (67945-1-Ig)

    WB analysis using 67945-1-Ig (same clone as 67945-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67945-1-Ig (FARSA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FARSA Monoclonal antibody (67945-1-Ig)

    WB analysis using 67945-1-Ig (same clone as 67945-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67945-1-Ig (FARSA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FARSA Monoclonal antibody (67945-1-Ig)

    IHC staining of human breast cancer using 67945-1-Ig (same clone as 67945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FARSA Monoclonal antibody (67945-1-Ig)

    IHC staining of human breast cancer using 67945-1-Ig (same clone as 67945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FARSA Monoclonal antibody (67945-1-Ig)

    IHC staining of human liver cancer using 67945-1-Ig (same clone as 67945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FARSA Monoclonal antibody (67945-1-Ig)

    IHC staining of human liver cancer using 67945-1-Ig (same clone as 67945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FARSA Monoclonal antibody (67945-1-Ig)

    IF Staining of A431 using 67945-1-Ig (same clone as 67945-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FARSA antibody (67945-1-Ig, Clone: 2D3E2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

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FARSA Monoclonal antibody, PBS Only

FARSA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67945-1-PBS
Clone No.2D3E2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FARSLA, FARSL, FARS, EC:6.1.1.20, CML33

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67945-1-PBS targets FARSA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FARSA fusion protein Ag12647 相同性解析による交差性が予測される生物種
Full Name phenylalanyl-tRNA synthetase, alpha subunit
Calculated molecular weight 58 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC006495
Gene Symbol FARSA
Gene ID (NCBI) 2193
RRIDAB_2918697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y285
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.