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  • Featured Product
  • KD/KO Validated

Fas/CD95 Monoclonal antibody, PBS Only

Fas/CD95 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G7F7

Cat no : 60196-1-PBS

Synonyms

ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS/CD95, FAS1, FASLG receptor, FASTM, TNFRSF6

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Fas/CD95 Monoclonal antibody (60196-1-Ig)

    WB analysis of Jurkat using 60196-1-Ig (same clone as 60196-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Fas/CD95 Monoclonal antibody (60196-1-Ig)

    WB analysis of HeLa using 60196-1-Ig (same clone as 60196-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using Fas/CD95 Monoclonal antibody (60196-1-Ig)

    WB analysis of K-562 using 60196-1-Ig (same clone as 60196-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using Fas/CD95 Monoclonal antibody (60196-1-Ig)

    WB analysis of U-937 using 60196-1-Ig (same clone as 60196-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Fas/CD95 Monoclonal antibody (60196-1-Ig)

    IHC staining of human tonsillitis using 60196-1-Ig (same clone as 60196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Fas/CD95 Monoclonal antibody (60196-1-Ig)

    IHC staining of human tonsillitis using 60196-1-Ig (same clone as 60196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Fas/CD95 Monoclonal antibody (60196-1-Ig)

    IHC staining of human colon cancer using 60196-1-Ig (same clone as 60196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Fas/CD95 Monoclonal antibody (60196-1-Ig)

    IHC staining of human colon cancer using 60196-1-Ig (same clone as 60196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60196-1-PBS targets Fas/CD95 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fas/CD95 fusion protein Ag3718 相同性解析による交差性が予測される生物種
Full Name Fas (TNF receptor superfamily, member 6)
Calculated molecular weight 35-38 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC012479
Gene symbol Fas/CD95
Gene ID (NCBI) 355
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). This anti-Fas monoclonal antibody can be used to induce apoptosis in cell cultures through Fas by imitating the Fas-ligand.