FASN Monoclonal antibody

FASN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B6E4

Cat no : 66591-1-Ig

Synonyms

FAS, FASN, fatty acid synthase, OA 519, SDR27X1


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Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66591-1-Ig targets FASN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, monkey
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FASN fusion protein Ag0975 相同性解析による交差性が予測される生物種
Full Name fatty acid synthase
Calculated molecular weight 272 kDa
Observed molecular weight 272 kDa
GenBank accession numberBC007909
Gene symbol FASN
Gene ID (NCBI) 2194
RRIDAB_2881951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

Protocols

Product Specific Protocols
WB protocol for FASN antibody 66591-1-IgDownload protocol
IHC protocol for FASN antibody 66591-1-IgDownload protocol
IF protocol for FASN antibody 66591-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai
humanWB

Theranostics

Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription.

Authors - Gengyi Zou
humanWB,IF,IP

J Transl Med

TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis

Authors - Xiang-Lin Hao
humanIHC,IP

Am J Cancer Res

Ubiquitin specific peptidase 38 promotes the progression of gastric cancer through upregulation of fatty acid synthase.

Authors - Zhi Zheng
humanWB

Environ Toxicol

SIRT1/mTOR pathway-mediated autophagy dysregulation promotes Pb-induced hepatic lipid accumulation in HepG2 cells

Authors - Yanan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sun (Verified Customer) (01-05-2023)

We detected a band around 200-300kDa region in HEK293T cells using this antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T