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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FASN Recombinant antibody, PBS Only

FASN Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1L9

Cat no : 81079-1-PBS

Synonyms

3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 1L9, [Acyl-carrier-protein] S-malonyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FASN Recombinant antibody (81079-1-RR)

    WB analysis using 81079-1-RR (same clone as 81079-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81079-1-RR (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81079-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FASN Recombinant antibody (81079-1-RR)

    IF Staining of HeLa using 81079-1-RR (same clone as 81079-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FASN antibody (81079-1-RR, Clone: 1L9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81079-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81079-1-RR

    Affinity and Kinetic Characterization of 81079-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81079-1-RR against Human FASN were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81079-1-PBS targets FASN in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FASN fusion protein Ag0975 相同性解析による交差性が予測される生物種
Full Name fatty acid synthase
Calculated molecular weight 272 kDa
Observed molecular weight250-270 kDa
GenBank accession numberBC007909
Gene symbol FASN
Gene ID (NCBI) 2194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.