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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FASN Recombinant antibody (81079-1-RR)

    WB analysis using 81079-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81079-1-RR (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FASN Recombinant antibody (81079-1-RR)

    IF Staining of HeLa using 81079-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FASN antibody (81079-1-RR, Clone: 1L9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81079-1-RR

    Affinity and Kinetic Characterization of 81079-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81079-1-RR against Human FASN were performed. The affinity constant is below 1 pM.

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FASN Recombinant antibody

FASN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81079-1-RR
Clone No.1L9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 1L9, [Acyl-carrier-protein] S-malonyltransferase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, U-251 cells, Neuro-2a cells, HSC-T6 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81079-1-RR targets FASN in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FASN fusion protein Ag0975 相同性解析による交差性が予測される生物種
Full Name fatty acid synthase
Calculated molecular weight 272 kDa
Observed molecular weight250-270 kDa
GenBank accession numberBC007909
Gene Symbol FASN
Gene ID (NCBI) 2194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49327
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

Protocols

Product Specific Protocols
WB protocol for FASN antibody 81079-1-RRDownload protocol
IF protocol for FASN antibody 81079-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols