FASN Recombinant antibody
FASN Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1L9
Cat no : 81079-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, U-251 cells, Neuro-2a cells, HSC-T6 cells, mouse liver tissue, rat liver tissue |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
81079-1-RR targets FASN in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | FASN fusion protein Ag0975 相同性解析による交差性が予測される生物種 |
Full Name | fatty acid synthase |
Calculated molecular weight | 272 kDa |
Observed molecular weight | 250-270 kDa |
GenBank accession number | BC007909 |
Gene symbol | FASN |
Gene ID (NCBI) | 2194 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.
Protocols
Product Specific Protocols | |
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WB protocol for FASN antibody 81079-1-RR | Download protocol |
IF protocol for FASN antibody 81079-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |