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  • Featured Product
  • KD/KO Validated

FASTKD3 Polyclonal antibody, PBS Only

FASTKD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18392-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FASTKD3 Polyclonal antibody (18392-1-AP)

    WB analysis of HeLa using 18392-1-AP (same clone as 18392-1-PBS)

    WB result of FASTKD3 antibody (18392-1-AP, 1:200) with HeLa and 293 cell lysate by Maciej Szewczyk University of Warsaw. This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FASTKD3 Polyclonal antibody (18392-1-AP)

    WB analysis of HepG2 using 18392-1-AP (same clone as 18392-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18392-1-AP (FASTKD3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FASTKD3 Polyclonal antibody (18392-1-AP)

    WB analysis of HeLa using 18392-1-AP (same clone as 18392-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18392-1-AP (FASTKD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FASTKD3 Polyclonal antibody (18392-1-AP)

    IHC staining of human breast cancer using 18392-1-AP (same clone as 18392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FASTKD3 Polyclonal antibody (18392-1-AP)

    IHC staining of human breast cancer using 18392-1-AP (same clone as 18392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of transfected cells using FASTKD3 Polyclonal antibody (18392-1-AP)

    IF Staining of transfected cells using 18392-1-AP (same clone as 18392-1-PBS)

    IF result of anti-FASTKD3 (18392-1-AP, 1:200) with transfected COS7 cell by Dr. Nancy Kedersha. This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FASTKD3 Polyclonal antibody (18392-1-AP)

    IF Staining of HepG2 using 18392-1-AP (same clone as 18392-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 18392-1-AP (FASTKD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FASTKD3 Polyclonal antibody (18392-1-AP)

    IF Staining of HeLa using 18392-1-AP (same clone as 18392-1-PBS)

    Immunofluorescent analysis of HeLa cells using 18392-1-AP (FASTKD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18392-1-PBS targets FASTKD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FASTKD3 fusion protein Ag13083 相同性解析による交差性が予測される生物種
Full Name FAST kinase domains 3
Calculated molecular weight 76 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC001295
Gene symbol FASTKD3
Gene ID (NCBI) 79072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FAST kinase domain-containing protein 3(FASTKD3) belongs to FASTKD family.FASTKD3 is an essential component of mitochondrial respiration that may modulate energy balance in cells exposed to adverse conditions by functionally coupling mitochondrial protein synthesis to respiration. The expression of endogenous FASTKD3 is very weak. This antibody detects the c-terminal of FASTKD3.