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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAT1 Polyclonal antibody

FAT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30733-1-AP

Synonyms

Cadherin family member 7, CDHF7, FAT, FAT1, hFat1, ME5, Protein fat homolog, Protocadherin Fat 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAT1 Polyclonal antibody (30733-1-AP)

    WB analysis using 30733-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30733-1-AP (FAT1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FAT1 Polyclonal antibody (30733-1-AP)

    WB analysis using 30733-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30733-1-AP (FAT1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using FAT1 Polyclonal antibody (30733-1-AP)

    IHC staining of human prostate hyperplasia using 30733-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 30733-1-AP (FAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using FAT1 Polyclonal antibody (30733-1-AP)

    IHC staining of human prostate hyperplasia using 30733-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 30733-1-AP (FAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30733-1-AP targets FAT1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAT1 fusion protein Ag33957 相同性解析による交差性が予測される生物種
Full Name FAT tumor suppressor homolog 1 (Drosophila)
Observed molecular weight506-600 kDa
Gene symbol FAT1
Gene ID (NCBI) 2195
RRIDAB_3086406
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAT1, also known as hFat1, belongs to a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. It is expected to be located in cell membrane and nucleus, which is expressed in many epithelial and some endothelial and smooth muscle cells. The calculated molecular weight of FAT1 is 506 kDa and there is glycosylation modification of the protein. To examine functions of the transmembrane and cytoplasmic domains, they were expressed in HEK-293 and HeLa cells as chimeric proteins in fusion with EGFP and extracellular domains derived from E-cadherin. Proteins comprising the transmembrane domain localized to the membrane fraction (PMID: 15922730, 26373379).

Protocols

Product Specific Protocols
WB protocol for FAT1 antibody 30733-1-APDownload protocol
IHC protocol for FAT1 antibody 30733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols