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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FAT1 Recombinant antibody (84279-3-RR)

    WB analysis of HeLa using 84279-3-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 84279-3-RR (FAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84279-3-RR

    Affinity and Kinetic Characterization of 84279-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84279-3-RR against Human FAT1 were performed. The affinity constant is 0.375 nM.

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FAT1 Recombinant antibody

FAT1 Recombinant Antibody for WB, ELISA
Cat No. 84279-3-RR
Clone No.241602F9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ME5, hFat1, FAT, CDHF7, 241602F9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84279-3-RR targets FAT1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FAT1 fusion protein Ag33957 相同性解析による交差性が予測される生物種
Full Name FAT tumor suppressor homolog 1 (Drosophila)
Observed molecular weight506-600 kDa
Gene Symbol FAT1
Gene ID (NCBI) 2195
RRIDAB_3671825
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14517
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAT1, also known as hFat1, belongs to a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. It is expected to be located in cell membrane and nucleus, which is expressed in many epithelial and some endothelial and smooth muscle cells. The calculated molecular weight of FAT1 is 506 kDa and there is glycosylation modification of the protein. To examine functions of the transmembrane and cytoplasmic domains, they were expressed in HEK-293 and HeLa cells as chimeric proteins in fusion with EGFP and extracellular domains derived from E-cadherin. Proteins comprising the transmembrane domain localized to the membrane fraction (PMID: 15922730, 26373379).

Protocols

Product Specific Protocols
WB protocol for FAT1 antibody 84279-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols