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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAT2 Polyclonal antibody (31490-1-AP)

    WB analysis using 31490-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31490-1-AP (FAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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FAT2 Polyclonal antibody

FAT2 Polyclonal Antibody for WB, ELISA
Cat No. 31490-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Protocadherin Fat 2, MEGF1, KIAA0811, HFAT2, CDHF8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31490-1-AP targets FAT2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAT2 fusion protein Ag34942 相同性解析による交差性が予測される生物種
Full Name FAT tumor suppressor homolog 2 (Drosophila)
Calculated molecular weight479 kDa
Observed molecular weight500-600 kDa
GenBank accession numberNM_001447
Gene Symbol FAT2
Gene ID (NCBI) 2196
RRIDAB_3670004
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NYQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAT2 (FAT atypical cadherin 2), also known as CDHF8. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. This protein most likely functions as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development. Expressed in epidermal keratinocytes, infant brain, cerebellum, and also in a variety of tumors, such as pancreatic cancer, diffuse type gastric cancer, ovarian cancer, esophageal cancer, skin squamous cell carcinoma, head and neck cancer. Not expressed in melanoma cell line A375 cells, normal epidermal melanocytes or normal dermal fibroblasts. Expressed in epidermal keratinocytes and squamous cell carcinoma (at protein level). The molecular weight of FAT2 is 473 kDa. The huge molecular masses (about 500-600 kDa) have hindered studies of the molecular aspects of itself roles(PMID: 15923647).

Protocols

Product Specific Protocols
WB protocol for FAT2 antibody 31490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols