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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FATP2 Monoclonal antibody (68074-1-Ig)

    WB analysis using 68074-1-Ig (same clone as 68074-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FATP2 Monoclonal antibody (68074-1-Ig)

    WB analysis of HepG2 using 68074-1-Ig (same clone as 68074-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using FATP2 Monoclonal antibody (68074-1-Ig)

    WB analysis of rat liver using 68074-1-Ig (same clone as 68074-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68074-1-Ig (FATP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FATP2 Monoclonal antibody (68074-1-Ig)

    IHC staining of human kidney using 68074-1-Ig (same clone as 68074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FATP2 Monoclonal antibody (68074-1-Ig)

    IHC staining of human kidney using 68074-1-Ig (same clone as 68074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FATP2 Monoclonal antibody (68074-1-Ig)

    IF Staining of HepG2 using 68074-1-Ig (same clone as 68074-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FATP2 antibody (68074-1-Ig, Clone: 2C10D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68074-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50832-1

    Cytometric bead array standard curve of MP50832-1

    Cytometric bead array standard curve of MP50832-1, SLC27A2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68074-1-PBS. Detection antibody: 68074-2-PBS. Standard:Ag5390. Range: 12.5-200 ng/mL.

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FATP2 Monoclonal antibody, PBS Only (Capture)

FATP2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68074-1-PBS
Clone No.2C10D7

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

SLC27A2, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-, Arachidonate--CoA ligase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68074-1-PBS targets FATP2 as part of a matched antibody pair:

MP50832-1: 68074-1-PBS capture and 68074-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FATP2 fusion protein Ag5390 相同性解析による交差性が予測される生物種
Full Name solute carrier family 27 (fatty acid transporter), member 2
Calculated molecular weight 567 aa, 65 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC057770
Gene Symbol FATP2/SLC27A2
Gene ID (NCBI) 11001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14975
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.