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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FATP5/SLC27A5 Recombinant antibody, PBS Only

FATP5/SLC27A5 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241884H2

Cat no : 84557-1-PBS

Synonyms

FATP5, SLC27A5, Bile acyl-CoA synthetase, Bile acid-CoA ligase, BA-CoA ligase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FATP5/SLC27A5 Recombinant antibody (84557-1-RR)

    WB analysis using 84557-1-RR (same clone as 84557-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84557-1-RR (SLC27A5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FATP5/SLC27A5 Recombinant antibody (84557-1-RR)

    IHC staining of mouse liver using 84557-1-RR (same clone as 84557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84557-1-RR (FATP5/SLC27A5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FATP5/SLC27A5 Recombinant antibody (84557-1-RR)

    IHC staining of mouse liver using 84557-1-RR (same clone as 84557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84557-1-RR (FATP5/SLC27A5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84557-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84557-1-PBS targets FATP5/SLC27A5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FATP5/SLC27A5 fusion protein Ag18276 相同性解析による交差性が予測される生物種
Full Name solute carrier family 27 (fatty acid transporter), member 5
Calculated molecular weight 690 aa, 75 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC148807
Gene symbol FATP5/SLC27A5
Gene ID (NCBI) 10998
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SLC27A5, also known as FATP5, ACSVL6, and BAL, is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty acids containing 24- and 26-carbons(PMID: 10479480). FATP5 mediates the import of long-chain fatty acids (LCFA) by facilitating their transport across cell membranes (PubMed:20448275). FATP5 is associated with endoplasmic reticulum but not with peroxisomes, and its primary role is in fatty acid elongation or complex lipid synthesis. FATP5 is exclusively expressed by the liver and localized to the basal plasma membrane of hepatocytes (PMID:16618416).