Validation Data Gallery
Tested Applications
Positive WB detected in | A375 cells, mouse liver tissue, mosue kidney tissue |
Positive IP detected in | A375 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 18 publications below |
IF | See 10 publications below |
IP | See 1 publications below |
RIP | See 4 publications below |
Product Information
16021-1-AP targets FBL in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FBL fusion protein Ag8841 相同性解析による交差性が予測される生物種 |
Full Name | fibrillarin |
Calculated molecular weight | 321 aa, 34 kDa |
Observed molecular weight | 34 kDa |
GenBank accession number | BC019260 |
Gene Symbol | FBL |
Gene ID (NCBI) | 2091 |
RRID | AB_2105788 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P22087 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for FBL antibody 16021-1-AP | Download protocol |
IHC protocol for FBL antibody 16021-1-AP | Download protocol |
IF protocol for FBL antibody 16021-1-AP | Download protocol |
IP protocol for FBL antibody 16021-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing | ||
Mol Cell LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.
| ||
Breast Cancer Res U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression | ||
Cell Biol Toxicol SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells | ||
Commun Biol Nascent ribosomal RNA act as surfactant that suppresses growth of fibrillar centers in nucleolus |