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FBL Polyclonal antibody

Name: FBL Polyclonal antibody
Brand: Proteintech
SKU: 16021-1-AP
Rating: 5.00 (4 reviews)

FBL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (4)

Cat no : 16021-1-AP

Synonyms

RNU3IP1, FLRN, fibrillarin, FIB1, FIB

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 16021-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16021-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16021-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 16021-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 16021-1-AP (FBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A375 using 16021-1-AP
IP result of anti-FBL (IP:16021-1-AP, 4ug; Detection:16021-1-AP 1:500) with A375 cells lysate 3600ug.
IHC staining of mouse brain using 16021-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16021-1-AP (FBL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 16021-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16021-1-AP (FBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16021-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 16021-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 16021-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (16021-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 16021-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A375 cells, mouse liver tissue, mosue kidney tissue
Positive IP detected in	A375 cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 17 publications below
IF	See 9 publications below
IP	See 1 publications below
RIP	See 3 publications below

Product Information

16021-1-AP targets FBL in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FBL fusion protein Ag8841 相同性解析による交差性が予測される生物種
Full Name	fibrillarin
Calculated molecular weight	321 aa, 34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC019260
Gene symbol	FBL
Gene ID (NCBI)	2091
RRID	AB_2105788
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for FBL antibody 16021-1-AP	Download protocol
IHC protocol for FBL antibody 16021-1-AP	Download protocol
IF protocol for FBL antibody 16021-1-AP	Download protocol
IP protocol for FBL antibody 16021-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Oxidative cyclization reagents reveal tryptophan cation-π interactions Authors - Xiao Xie View Article
human	WB	Mol Cell LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice. Authors - Xin Wang KD Validated View Article
human	WB	Breast Cancer Res U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression Authors - Wenrong Zhang View Article
human	WB,IF	Cell Biol Toxicol SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells Authors - Jingyi Cui View Article
	IF	Commun Biol Nascent ribosomal RNA act as surfactant that suppresses growth of fibrillar centers in nucleolus Authors - Tetsuya Yamamoto View Article
human	WB	Oncotarget Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis. Authors - Xing Liu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Tatyana (Verified Customer) (03-11-2023)	Very strong, specific nucleolar signal in ICC. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h RT incubation (no blocking step). Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human, U2OS cells
FH WEI (Verified Customer) (03-12-2020)	Good, strong band for WB and correct staining for cardiomyocytes. Applications: Western Blot, Immunofluorescence, Cell Tissue Type: Mouse Heart
FH Lauren (Verified Customer) (02-21-2020)	Fixations tested include 2% and 4% formaldehyde for 20 minutes, and -20'C methanol for 10 minutes. Antibody works in all three fixations, but 4% formaldehyde gave the cleanest signal. Applications: Immunofluorescence, Primary Antibody Dilution: 1:100 Cell Tissue Type: mIMCD3 FBL Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:16021-1-AP)
FH Baptiste (Verified Customer) (09-06-2019)	This antibody works well in western blot Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: human satelllite muscle cells FBL Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:16021-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBL Polyclonal antibody

FBL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 16021-1-AP

WB analysis of mouse kidney using 16021-1-AP

WB analysis of mouse liver using 16021-1-AP

WB analysis of A375 using 16021-1-AP

IP experiment of A375 using 16021-1-AP

IHC staining of mouse brain using 16021-1-AP

IHC staining of mouse brain using 16021-1-AP

IF Staining of HepG2 using 16021-1-AP

IF Staining of HepG2 using 16021-1-AP

IF Staining of HepG2 using 16021-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Oxidative cyclization reagents reveal tryptophan cation-π interactions

LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.

U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression

SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells

Nascent ribosomal RNA act as surfactant that suppresses growth of fibrillar centers in nucleolus

Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis.

Reviews