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KD/KO Validated

Fibulin 5 Polyclonal antibody

Name: Fibulin 5 Polyclonal antibody
Brand: Proteintech
SKU: 12188-1-AP

Fibulin 5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12188-1-AP

Synonyms

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein

Validation Data Gallery

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AllWBIPIHC

WB analysis using 12188-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 12188-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 12188-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 12188-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 12188-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 12188-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12188-1-AP (Fibulin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 12188-1-AP
IP result of anti-Fibulin 5 (IP:12188-1-AP, 3ug; Detection:12188-1-AP 1:300) with mouse heart tissue lysate 8000ug.
IHC staining of mouse lung using 12188-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12188-1-AP (Fibulin 5 antibody at dilution of 1:200.
IHC staining of mouse lung using 12188-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12188-1-AP (Fibulin 5 antibody at dilution of 1:200.
IHC staining of human heart using 12188-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 12188-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 12188-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 12188-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12188-1-AP (Fibulin 5 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 12188-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse heart tissue, rat brain tissue, mouse placenta tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	mouse lung tissue, human heart tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

12188-1-AP targets Fibulin 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Fibulin 5 fusion protein Ag2859 相同性解析による交差性が予測される生物種
Full Name	fibulin 5
Calculated molecular weight	448 aa, 50 kDa
Observed molecular weight	55 kDa, 65 kDa
GenBank accession number	BC022280
Gene symbol	Fibulin 5
Gene ID (NCBI)	10516
RRID	AB_2105939
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein.

Protocols

Product Specific Protocols
WB protocol for Fibulin 5 antibody 12188-1-AP	Download protocol
IHC protocol for Fibulin 5 antibody 12188-1-AP	Download protocol
IP protocol for Fibulin 5 antibody 12188-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Arterioscler Thromb Vasc Biol Increased aortic stiffness and attenuated lysyl oxidase activity in obesity. Authors - Chen Ju-Yi JY View Article
human	WB	Biochem J Fibulin-5 binds urokinase-type plasminogen activator and mediates urokinase-stimulated β1-integrin-dependent cell migration. Authors - Kapustin Alexander A View Article
mouse	WB, IHC	Cell Tissue Res Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin. Authors - Wenli Han View Article
mouse	WB	Am J Physiol Cell Physiol Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function. Authors - Varadarajan Sudhahar View Article
human	WB	Clin Exp Pharmacol Physiol Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway. Authors - Wei Zhang KD Validated View Article
human	IF	JID Innov Fibulin-4 Accelerates Amyloid Formation by Binding with a Keratin 5 Peptide Fragment. Authors - Fumihiko Katagiri View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Fibulin 5 Polyclonal antibody

Fibulin 5 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 12188-1-AP

WB analysis using 12188-1-AP

WB analysis of mouse skeletal muscle using 12188-1-AP

WB analysis of mouse heart using 12188-1-AP

WB analysis of rat brain using 12188-1-AP

WB analysis of mouse placenta using 12188-1-AP

IP experiment of mouse heart using 12188-1-AP

IHC staining of mouse lung using 12188-1-AP

IHC staining of mouse lung using 12188-1-AP

IHC staining of human heart using 12188-1-AP

IHC staining of human heart using 12188-1-AP

IHC staining of human pancreas using 12188-1-AP

IHC staining of human pancreas using 12188-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Increased aortic stiffness and attenuated lysyl oxidase activity in obesity.

Fibulin-5 binds urokinase-type plasminogen activator and mediates urokinase-stimulated β1-integrin-dependent cell migration.

Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin.

Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function.

Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway.

Fibulin-4 Accelerates Amyloid Formation by Binding with a Keratin 5 Peptide Fragment.