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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Fibrillin 1 Recombinant antibody, PBS Only (Capture)

Fibrillin 1 Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240960E11

Cat no : 83836-2-PBS

Synonyms

FBN1, MASS, Fibrillin-1, FBN, Asprosin

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00807-1

    Sandwich ELISA standard curve of MP00807-1

    Sandwich ELISA standard curve of MP00807-1, Human FBN1 Recombinant Matched Antibody Pair - PBS only. 83836-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30283. 83836-1-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

  • Sample test of MP00807-1

    Sample test of MP00807-1

    Serum of sixteen individual healthy human donors was measured. The human FBN1 concentration of detected samples was determined to be 117.73 ng/mL with a range of 43.48 - 246.44 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Fibrillin 1 Recombinant antibody (83836-2-RR)

    IF Staining of HeLa using 83836-2-RR (same clone as 83836-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Fibrillin 1 antibody (83836-2-RR, Clone: 240960E11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83836-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00807-1

    Cytometric bead array standard curve of MP00807-1

    Cytometric bead array standard curve of MP00807-1, Fibrillin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83836-2-PBS. Detection antibody: 83836-1-PBS. Standard: Ag30283. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83836-2-RR

    Affinity and Kinetic Characterization of 83836-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83836-2-RR against Human Fibrillin 1 were performed. The affinity constant is 0.103 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83836-2-PBS targets Fibrillin 1 as part of a matched antibody pair:

MP00807-1: 83836-2-PBS capture and 83836-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fibrillin 1 fusion protein Ag30283 相同性解析による交差性が予測される生物種
Full Name fibrillin 1
GenBank accession numberBC146854
Gene symbol FBN1
Gene ID (NCBI) 2200
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fibrillin-1 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Defects in Fibrillin-1 are a cause of Marfan syndrome (MFS), isolated ectopia lentis (EL), Weill-Marchesani syndrome autosomal dominant (ADWMS), Shprintzen-Goldberg craniosynostosis syndrome (SGS) or MASS syndrome.