fibrillin 2-Specific Polyclonal antibody

Name: fibrillin 2-Specific Polyclonal antibody
Brand: Proteintech
SKU: 20252-1-AP

fibrillin 2-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20252-1-AP

Synonyms

CCA, DA9, FBN2, fibrillin 2, fibrillin 2-Specific

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of human brain using 20252-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 20252-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20252-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20252-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 20252-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 20252-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 20252-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 20252-1-AP (fibrillin 2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 20252-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 20252-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 20252-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:200 (under 10x lens).
IHC staining of human placenta using 20252-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20252-1-AP (fibrillin 2-Specific antibody at dilution of 1:200 (under 40x lens).

-/ -

Cat No. 20252-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human brain tissue, HepG2 cells, L02 cells, mouse lung tissue
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

20252-1-AP targets fibrillin 2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	fibrillin 2
Calculated molecular weight	315 kDa
Observed molecular weight	290 kDa, 160 kDa
GenBank accession number	NM_001999
Gene symbol	Fibrillin 2
Gene ID (NCBI)	2201
RRID	AB_11182265
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FBN2 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils which occur either in association with elastin or in elastin-free bundles. Fibrillin-2-containing microfibrils regulate the early process of elastic fiber assembly. Defects in FBN2 are the cause of congenital contractural arachnodactyly (CCA) which also known as Beals syndrome or distal arthrogryposis type 9 (DA9). This antibody is specific to FBN2. It recognizes both two isoforms ~300 kDa and 160 kDa.

Protocols

Product Specific Protocols
WB protocol for fibrillin 2-Specific antibody 20252-1-AP	Download protocol
IHC protocol for fibrillin 2-Specific antibody 20252-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Head Neck High expression of NFX1-123 in HPV positive head and neck squamous cell carcinomas. Authors - Sreenivasulu Chintala View Article
mouse	IHC	J Ovarian Res hUMSC transplantation restores follicle development in ovary damaged mice via re-establish extracellular matrix (ECM) components Authors - Yin Shuyuan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

fibrillin 2-Specific Polyclonal antibody

fibrillin 2-Specific Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of human brain using 20252-1-AP

WB analysis of human brain using 20252-1-AP

WB analysis of HepG2 using 20252-1-AP

WB analysis of HepG2 using 20252-1-AP

WB analysis of mouse lung using 20252-1-AP

WB analysis of L02 using 20252-1-AP

WB analysis of L02 using 20252-1-AP

IHC staining of human placenta using 20252-1-AP

IHC staining of human placenta using 20252-1-AP

IHC staining of human placenta using 20252-1-AP

IHC staining of human placenta using 20252-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

High expression of NFX1-123 in HPV positive head and neck squamous cell carcinomas.

hUMSC transplantation restores follicle development in ovary damaged mice via re-establish extracellular matrix (ECM) components