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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using FBP17 Polyclonal antibody (27056-1-AP)

    WB analysis of mouse brain using 27056-1-AP (same clone as 27056-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27056-1-AP (FBP17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27056-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using FBP17 Polyclonal antibody (27056-1-AP)

    IP experiment of mouse brain using 27056-1-AP (same clone as 27056-1-PBS)

    IP result of anti-FBP17 (IP:27056-1-AP, 4ug; Detection:27056-1-AP 1:1000) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 27056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FBP17 Polyclonal antibody (27056-1-AP)

    IHC staining of human lung cancer using 27056-1-AP (same clone as 27056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FBP17 Polyclonal antibody (27056-1-AP)

    IHC staining of human lung cancer using 27056-1-AP (same clone as 27056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FBP17 Polyclonal antibody (27056-1-AP)

    IHC staining of human breast cancer using 27056-1-AP (same clone as 27056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using FBP17 Polyclonal antibody (27056-1-AP)

    IHC staining of mouse heart using 27056-1-AP (same clone as 27056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using FBP17 Polyclonal antibody (27056-1-AP)

    IHC staining of mouse heart using 27056-1-AP (same clone as 27056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using FBP17 Polyclonal antibody (27056-1-AP)

    IHC staining of mouse heart using 27056-1-AP (same clone as 27056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using FBP17 Polyclonal antibody (27056-1-AP)

    IHC staining of mouse heart using 27056-1-AP (same clone as 27056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FBP17 Polyclonal antibody (27056-1-AP)

    IHC staining of human breast cancer using 27056-1-AP (same clone as 27056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27056-1-PBS in a different storage buffer formulation.

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FBP17 Polyclonal antibody, PBS Only

FBP17 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27056-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27056-1-PBS targets FBP17 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBP17 fusion protein Ag25784 相同性解析による交差性が予測される生物種
Full Name formin binding protein 1
Observed molecular weight 80-85 kDa
GenBank accession numberBC101755
Gene Symbol FBP17
Gene ID (NCBI) 23048
RRIDAB_2880734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RU3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FBP17 (formin-binding protein 17) is a new dynamin-associating molecule consisting of several functional domains, including an N-terminal EFC domain and an SH3 domain at the C-terminus. FBP17 is involved in dynamin-mediated endocytosis and has been reported to be required for invadopodia formation and tumor cell invasion.