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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBP2 Recombinant antibody

FBP2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240879C9

Cat no : 83910-5-RR

Synonyms

Muscle FBPase, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, EC:3.1.3.11, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using FBP2 Recombinant antibody (83910-5-RR)

    WB analysis of mouse heart using 83910-5-RR

    Mouse heart tissue were subjected to SDS PAGE followed by western blot with 83910-5-RR (FBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using FBP2 Recombinant antibody (83910-5-RR)

    WB analysis of rat skeletal muscle using 83910-5-RR

    Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 83910-5-RR (FBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FBP2 Recombinant antibody (83910-5-RR)

    IHC staining of human ovary cancer using 83910-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FBP2 Recombinant antibody (83910-5-RR)

    IHC staining of mouse skeletal muscle using 83910-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FBP2 Recombinant antibody (83910-5-RR)

    IHC staining of mouse skeletal muscle using 83910-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using FBP2 Recombinant antibody (83910-5-RR)

    IF Staining of mouse skeletal muscle using 83910-5-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBP2 antibody (83910-5-RR, Clone: 240879C9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83910-5-RR

    Affinity and Kinetic Characterization of 83910-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83910-5-RR against Human FBP2 were performed. The affinity constant is 4.67 nM.

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Tested Applications

Positive WB detected inmouse heart tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83910-5-RR is FBP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FBP2 fusion protein Ag18245 相同性解析による交差性が予測される生物種
Full Name fructose-1,6-bisphosphatase 2
Calculated molecular weight 339 aa, 37 kDa
Observed molecular weight37 kDa
GenBank accession numberBC113632
Gene symbol FBP2
Gene ID (NCBI) 8789
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBP2, also named muscle FBP, belongs to the FBPase class 1 family. FBP2 is a ubiquitously expressed enzyme of glycogen synthesis from non-carbohydrates, e.g., lactate (glyconeogenesis). However, it also plays a variety of non-enzymatic functions. FBP2 is involved in the regulation of hypoxia-inducible factor 1 (Hif1) stability, cell cycle-dependent events, biogenesis of mitochondria, and protection of the organelles against high reactive oxygen species (ROS)- and high Ca2+-induced stress (PMID: 32962293, PMID: 35626746). The calculated molecular weight of FBP2 is 37 kDa.

Protocols

Product Specific Protocols
WB protocol for FBP2 antibody 83910-5-RRDownload protocol
IHC protocol for FBP2 antibody 83910-5-RRDownload protocol
IF protocol for FBP2 antibody 83910-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols