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Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using FBXL15 Polyclonal antibody (20895-1-AP)

    WB analysis of MDA-MB-453s using 20895-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20895-1-AP (FBXL15 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FBXL15 Polyclonal antibody (20895-1-AP)

    IHC staining of human breast cancer using 20895-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20895-1-AP (FBXL15 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FBXL15 Polyclonal antibody (20895-1-AP)

    IHC staining of human breast cancer using 20895-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20895-1-AP (FBXL15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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FBXL15 Polyclonal antibody

FBXL15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20895-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

F box only protein 37, F box/LRR repeat protein 15, Fbl15, FBXL15, FBXO37, JET, PSD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20895-1-AP targets FBXL15 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXL15 fusion protein Ag14949 相同性解析による交差性が予測される生物種
Full Name F-box and leucine-rich repeat protein 15
Calculated molecular weight 300 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC002912
Gene Symbol FBXL15
Gene ID (NCBI) 79176
RRIDAB_2878758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H469
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXL15, also known as FBXO37, belongs to the FBXL15 family. FBXL15 plays a role in the G2/M transition of the cell cycle, as well as in cellular protein metabolic processes and the positive regulation of the bone morphogenetic proteins signaling pathway (PMID: 38711244). The calculated molecular weight of FBXL15 is 33 kDa.

Protocols

Product Specific Protocols
WB protocol for FBXL15 antibody 20895-1-APDownload protocol
IHC protocol for FBXL15 antibody 20895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols