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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXL4 Polyclonal antibody (24754-1-AP)

    WB analysis using 24754-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24754-1-AP (FBXL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FBXL4 Polyclonal antibody (24754-1-AP)

    IF Staining of U2OS using 24754-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXL4 antibody (24754-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

FBXL4 Polyclonal antibody

FBXL4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24754-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FBL5, FBL4, F box/LRR repeat protein 4, F box protein FBL4/FBL5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse heart tissue, rat heart tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24754-1-AP targets FBXL4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXL4 fusion protein Ag21579 相同性解析による交差性が予測される生物種
Full Name F-box and leucine-rich repeat protein 4
Calculated molecular weight 621 aa, 70 kDa
Observed molecular weight70 kDa
GenBank accession numberBC055010
Gene Symbol FBXL4
Gene ID (NCBI) 26235
RRIDAB_3669448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXL4 (F-box and leucine-rich repeat protein 4) is a mitochondrial protein that has been recently found to play significant roles in mtDNA maintenance (PMID: 28940506). Moreover, FBXL4 is a substrate receptor of a Cullin-1-RING E3 (CRL1) ligase complex that has previously been shown to bind to SKP1, the common CRL1 adapter subunit (PMID: 37102372).

Protocols

Product Specific Protocols
WB protocol for FBXL4 antibody 24754-1-APDownload protocol
IF protocol for FBXL4 antibody 24754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols