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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

FBXO11 Polyclonal antibody

FBXO11 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 27610-1-AP

Synonyms

F box only protein 11, F box protein 11, FBX11, FBXO11, PRMT9, UBR6, UG063H01, VIT 1, VIT1, Vitiligo associated protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO11 Polyclonal antibody (27610-1-AP)

    WB analysis using 27610-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27610-1-AP (FBXO11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of LNCaP cells using FBXO11 Polyclonal antibody (27610-1-AP)

    IP experiment of LNCaP using 27610-1-AP

    IP result of anti-FBXO11 (IP:27610-1-AP, 4ug; Detection:27610-1-AP 1:500) with LNCaP cells lysate 2400 ug.

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Tested Applications

Positive WB detected inLNCaP cells, U2OS cells
Positive IP detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27610-1-AP targets FBXO11 in WB, IP, CoIP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO11 fusion protein Ag24401 相同性解析による交差性が予測される生物種
Full Name F-box protein 11
Calculated molecular weight 927 aa, 104 kDa
Observed molecular weight 94-104 kDa
GenBank accession numberBC012728
Gene symbol FBXO11
Gene ID (NCBI) 80204
RRIDAB_2880925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FBXO11 antibody 27610-1-APDownload protocol
IP protocol for FBXO11 antibody 27610-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FEBS Open Bio

The F-box protein FBXO11 restrains hepatocellular carcinoma stemness via promotion of ubiquitin-mediated degradation of Snail.

Authors - Lijiang Shao
  • KD Validated
View Article
humanWB,CoIP

Clin Transl Med

Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma

Authors - Hao Zhang
  • KD Validated
View Article