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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO17 Monoclonal antibody

FBXO17 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3D3

Cat no : 68246-1-Ig

Synonyms

F box only protein 17, F box only protein 26, F box protein 17, FBG4, Fbx17, FBX26, FBXO17, FBXO26, FLJ11798, FLJ25205, FLJ58300

Validation Data Gallery

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  • Western Blot (WB) analysis of NCI-H1299 cells using FBXO17 Monoclonal antibody (68246-1-Ig)

    WB analysis of NCI-H1299 using 68246-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using FBXO17 Monoclonal antibody (68246-1-Ig)

    WB analysis of A549 using 68246-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FBXO17 Monoclonal antibody (68246-1-Ig)

    WB analysis using 68246-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FBXO17 Monoclonal antibody (68246-1-Ig)

    IF Staining of HepG2 using 68246-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FBXO17 antibody (68246-1-Ig, Clone: 1F3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inNCI-H1299 cells, U2OS cells, A549 cells, SH-SY5Y cells, U-87 MG cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68246-1-Ig targets FBXO17 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FBXO17 fusion protein Ag3867 相同性解析による交差性が予測される生物種
Full Name F-box protein 17
Calculated molecular weight 278 aa, 31 kDa
Observed molecular weight29-31 kDa
GenBank accession numberBC012385
Gene symbol FBXO17
Gene ID (NCBI) 115290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

Protocols

Product Specific Protocols
WB protocol for FBXO17 antibody 68246-1-IgDownload protocol
IF protocol for FBXO17 antibody 68246-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols