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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FBXO18 Polyclonal antibody (14275-1-AP)

    WB analysis of HeLa using 14275-1-AP (same clone as 14275-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14275-1-AP (FBXO18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14275-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using FBXO18 Polyclonal antibody (14275-1-AP)

    IF Staining of PC-3 using 14275-1-AP (same clone as 14275-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 14275-1-AP (FBXO18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14275-1-PBS in a different storage buffer formulation.

FBXO18 Polyclonal antibody, PBS Only

FBXO18 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 14275-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DNA 3'-5' helicase 1, EC:5.6.2.4, FBH1, F-box DNA helicase 1, F-box only protein 18

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14275-1-PBS targets FBXO18 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5622

Product name: Recombinant human FBXO18 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 693-1043 aa of BC032674

Sequence: AEDKELEAKIAVVEKYNIRIPELVQRIEKCHIEDLDFAEYILGTVHKAKGLEFDTVHVLDDFVKVPCARHNLPQLPHFRVESFSEDEWNLLYVAVTRAKKRLIMTKSLENILTLAGEYFLQAELTSNVLKTGVVRCCVGQCNNAIPVDTVLTMKKLPITYSNRKENKGGYLCHSCAEQRIGPLAFLTASPEQVRAMERTVENIVLPRHEALLFLVF

相同性解析による交差性が予測される生物種
Full Name F-box protein, helicase, 18
Calculated molecular weight 118 kDa, 123 kDa
Observed molecular weight 118-123 kDa
GenBank accession numberBC032674
Gene Symbol FBXO18
Gene ID (NCBI) 84893
RRIDAB_10638916
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFZ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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