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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO32 Polyclonal antibody

FBXO32 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 28541-1-AP

Synonyms

Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FBXO32 Polyclonal antibody (28541-1-AP)

    IHC staining of mouse skeletal muscle using 28541-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28541-1-AP (FBXO32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FBXO32 Polyclonal antibody (28541-1-AP)

    IHC staining of mouse skeletal muscle using 28541-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28541-1-AP (FBXO32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28541-1-AP targets FBXO32 in IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO32 fusion protein Ag29410 相同性解析による交差性が予測される生物種
Full Name F-box protein 32
Calculated molecular weight 355 aa, 42 kDa
GenBank accession numberBC024030
Gene symbol FBXO32
Gene ID (NCBI) 114907
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.

Protocols

Product Specific Protocols
IHC protocol for FBXO32 antibody 28541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols