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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO32 Monoclonal antibody, PBS Only

FBXO32 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rat, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C2G3

Cat no : 67172-1-PBS

Synonyms

Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO32 Monoclonal antibody (67172-1-Ig)

    WB analysis using 67172-1-Ig (same clone as 67172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    WB analysis of human heart using 67172-1-Ig (same clone as 67172-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    WB analysis of pig heart using 67172-1-Ig (same clone as 67172-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    WB analysis of pig skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IHC staining of rat skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IHC staining of rat skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IHC staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IHC staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IF Staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IF Staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67172-1-PBS targets FBXO32 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, Rat, mouse samples.

Tested Reactivity Human, Pig, Rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FBXO32 fusion protein Ag25247 相同性解析による交差性が予測される生物種
Full Name F-box protein 32
Calculated molecular weight 355 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC024030
Gene symbol FBXO32
Gene ID (NCBI) 114907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.