• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO32 Recombinant antibody, PBS Only

FBXO32 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230243H7

Cat no : 82941-1-PBS

Synonyms

F-box only protein 32, F box protein 32, F box only protein 32, Atrogin-1, Atrogin 1

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FBXO32 Recombinant antibody (82941-1-RR)

    IHC staining of mouse skeletal muscle using 82941-1-RR (same clone as 82941-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82941-1-RR (FBXO32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82941-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using FBXO32 Recombinant antibody (82941-1-RR)

    IF Staining of C2C12 using 82941-1-RR (same clone as 82941-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using FBXO32 antibody (82941-1-RR, Clone: 230243H7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82941-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using FBXO32 Recombinant antibody (82941-1-RR)

    FC experiment of U-251 using 82941-1-RR (same clone as 82941-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human FBXO32 (82941-1-RR, Clone:230243H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82941-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82941-1-RR

    Affinity and Kinetic Characterization of 82941-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82941-1-RR against Human FBXO32 were performed. The affinity constant is below 1 pM.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82941-1-PBS targets FBXO32 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FBXO32 fusion protein Ag29394 相同性解析による交差性が予測される生物種
Full Name F-box protein 32
Calculated molecular weight 355 aa, 42 kDa
GenBank accession numberBC024030
Gene symbol FBXO32
Gene ID (NCBI) 114907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.