Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells, Jurkat cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
83509-6-RR targets FBXO38 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | FBXO38 fusion protein Ag35444 相同性解析による交差性が予測される生物種 |
| Full Name | F-box protein 38 |
| Calculated molecular weight | 134kDa，1188aa |
| Observed molecular weight | 133 kDa |
| GenBank accession number | XM_005268513.1 |
| Gene Symbol | FBXO38 |
| Gene ID (NCBI) | 81545 |
| RRID | AB_3671134 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q6PIJ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBXO38, also known as HMN2D, MOKA, and SP329, is a protein that plays a significant role in the regulation of immune responses, particularly in the context of cancer and autoimmune diseases. FBXO38 has been identified as an E3 ubiquitin ligase that mediates the degradation of PD-1 (Programmed cell death protein 1), a key immune checkpoint protein expressed on the surface of T cells. The internalization, ubiquitination, and proteasome degradation of PD-1 are facilitated by FBXO38, which links PD-1 to Lys48, leading to its poly-ubiquitination and subsequent degradation (PMID: 30487606).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO38 antibody 83509-6-RR | Download protocol |
| FC protocol for FBXO38 antibody 83509-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Identification and experimental validation of hub genes underlying depressive-like behaviors induced by chronic social defeat stress |