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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO44 Polyclonal antibody (10626-1-AP)

    WB analysis using 10626-1-AP (same clone as 10626-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10626-1-AP (FBXO44 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FBXO44 Polyclonal antibody (10626-1-AP)

    IHC staining of mouse brain using 10626-1-AP (same clone as 10626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10626-1-AP (FBXO44 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FBXO44 Polyclonal antibody (10626-1-AP)

    IHC staining of mouse brain using 10626-1-AP (same clone as 10626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10626-1-AP (FBXO44 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10626-1-PBS in a different storage buffer formulation.

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FBXO44 Polyclonal antibody, PBS Only

FBXO44 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10626-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10626-1-PBS targets FBXO44 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO44 fusion protein Ag0977 相同性解析による交差性が予測される生物種
Full Name F-box protein 44
Calculated molecular weight 26 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC007832
Gene Symbol FBXO44
Gene ID (NCBI) 93611
RRIDAB_2278465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4M3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)