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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO44 Polyclonal antibody

FBXO44 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30162-1-AP

Synonyms

DKFZp781J0852, F box only protein 44, F box protein 44, F box protein FBX30, F box/G domain protein 3, FBG3, FBX30, Fbx44, FBX6A, FBXO44, Fbxo6a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO44 Polyclonal antibody (30162-1-AP)

    WB analysis using 30162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30162-1-AP (FBXO44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FBXO44 Polyclonal antibody (30162-1-AP)

    IHC staining of mouse brain using 30162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30162-1-AP (FBXO44 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FBXO44 Polyclonal antibody (30162-1-AP)

    IHC staining of mouse brain using 30162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30162-1-AP (FBXO44 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30162-1-AP targets FBXO44 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO44 fusion protein Ag32432 相同性解析による交差性が予測される生物種
Full Name F-box protein 44
Calculated molecular weight 26 kDa
Observed molecular weight35 kDa
GenBank accession numberBC007832
Gene symbol FBXO44
Gene ID (NCBI) 93611
RRIDAB_3086253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)

Protocols

Product Specific Protocols
WB protocol for FBXO44 antibody 30162-1-APDownload protocol
IHC protocol for FBXO44 antibody 30162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols