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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO44 Recombinant antibody, PBS Only

FBXO44 Recombinant Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230229G7

Cat no : 82857-2-PBS

Synonyms

DKFZp781J0852, F box only protein 44, F box protein 44, F box protein FBX30, F box/G domain protein 3, FBG3, FBX30, Fbx44, FBX6A, FBXO44, Fbxo6a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO44 Recombinant antibody (82857-2-RR)

    WB analysis using 82857-2-RR (same clone as 82857-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82857-2-RR (FBXO44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82857-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FBXO44 Recombinant antibody (82857-2-RR)

    IHC staining of mouse brain using 82857-2-RR (same clone as 82857-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82857-2-RR (FBXO44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82857-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FBXO44 Recombinant antibody (82857-2-RR)

    IHC staining of mouse brain using 82857-2-RR (same clone as 82857-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82857-2-RR (FBXO44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82857-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FBXO44 Recombinant antibody (82857-2-RR)

    IF Staining of A431 using 82857-2-RR (same clone as 82857-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FBXO44 antibody (82857-2-RR, Clone: 230229G7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82857-2-PBS in a different storage buffer formulation.

  • Affinity experiments of 82857-2-RR

    Affinity experiments of 82857-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82857-2-RR against Human FBXO44 were performed. The affinity constant is 0.134 nM.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82857-2-PBS targets FBXO44 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FBXO44 fusion protein Ag0977 相同性解析による交差性が予測される生物種
Full Name F-box protein 44
Calculated molecular weight 26 kDa
Observed molecular weight30 kDa
GenBank accession numberBC007832
Gene symbol FBXO44
Gene ID (NCBI) 93611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)