FBXO5 Polyclonal antibody

Name: FBXO5 Polyclonal antibody
Brand: Proteintech
SKU: 10872-1-AP

FBXO5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10872-1-AP

Synonyms

Early mitotic inhibitor 1, EMI1, F box only protein 5, F box protein 5, FBX5, FBXO5

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of HEK-293 using 10872-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human ovary using 10872-1-AP
human ovary tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10872-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10872-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10872-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10872-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 10872-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 10872-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human placenta using 10872-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human placenta using 10872-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.
IHC staining of human placenta using 10872-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.
IHC staining of human tonsillitis using 10872-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.
IHC staining of human tonsillitis using 10872-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50.

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Cat No. 10872-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, human ovary tissue, K-562 cells, mouse kidney tissue, rat liver tissue
Positive IHC detected in	human placenta tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below

Product Information

10872-1-AP targets FBXO5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FBXO5 fusion protein Ag1296 相同性解析による交差性が予測される生物種
Full Name	F-box protein 5
Calculated molecular weight	54 kDa
Observed molecular weight	56 kDa, 45 kDa
GenBank accession number	BC018905
Gene symbol	FBXO5
Gene ID (NCBI)	26271
RRID	AB_2247034
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.

Protocols

Product Specific Protocols
WB protocol for FBXO5 antibody 10872-1-AP	Download protocol
IHC protocol for FBXO5 antibody 10872-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature CRL4AMBRA1 is a master regulator of D-type cyclins. Authors - Daniele Simoneschi View Article
human	WB	Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation Authors - Xiaohua Xu View Article
human	WB	J Cell Mol Med An upstream open reading frame regulates vasculogenic mimicry of glioma via ZNRD1-AS1/miR-499a-5p/ELF1/EMI1 pathway. Authors - Mo Wang View Article
human	WB	J Cancer Res Clin Oncol Identification of aberrantly expressed F-box proteins in squamous-cell lung carcinoma. Authors - Kai Wang KD Validated View Article
human	WB	Exp Cell Res The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation. Authors - Pan Wei View Article
human	WB	Mol Cell Probes Exploring the role of FBXO5 in gastric cancer Authors - Junchang Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO5 Polyclonal antibody

FBXO5 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 10872-1-AP

WB analysis of human ovary using 10872-1-AP

WB analysis of HeLa using 10872-1-AP

WB analysis of HepG2 using 10872-1-AP

WB analysis of K-562 using 10872-1-AP

WB analysis of mouse kidney using 10872-1-AP

WB analysis of rat liver using 10872-1-AP

IHC staining of human placenta using 10872-1-AP

IHC staining of human placenta using 10872-1-AP

IHC staining of human placenta using 10872-1-AP

IHC staining of human placenta using 10872-1-AP

IHC staining of human tonsillitis using 10872-1-AP

IHC staining of human tonsillitis using 10872-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CRL4AMBRA1 is a master regulator of D-type cyclins.

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

An upstream open reading frame regulates vasculogenic mimicry of glioma via ZNRD1-AS1/miR-499a-5p/ELF1/EMI1 pathway.

Identification of aberrantly expressed F-box proteins in squamous-cell lung carcinoma.

The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation.

Exploring the role of FBXO5 in gastric cancer