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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO7 Polyclonal antibody, PBS Only

FBXO7 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10696-1-PBS

Synonyms

PARK15, FBXO 7, FBX7, FBX07, FBX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO7 Polyclonal antibody (10696-1-AP)

    WB analysis using 10696-1-AP (same clone as 10696-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10696-1-AP (FBXO7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FBXO7 Polyclonal antibody (10696-1-AP)

    IHC staining of human stomach using 10696-1-AP (same clone as 10696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10696-1-AP (FBXO7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using FBXO7 Polyclonal antibody (10696-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 10696-1-AP (same clone as 10696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10696-1-AP (FBXO7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10696-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10696-1-PBS targets FBXO7 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO7 fusion protein Ag1035 相同性解析による交差性が予測される生物種
Full Name F-box protein 7
Calculated molecular weight 59 kDa
Observed molecular weight 59-75 kDa
GenBank accession numberBC008361
Gene symbol FBXO7
Gene ID (NCBI) 25793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FBXO7, also named as FBX7, is a substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO7 recognizes BIRC2 and DLGAP5. It function in phosphorylation-dependent ubiquitination.