Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30458-1-AP targets FBXO7 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FBXO7 fusion protein Ag32796 相同性解析による交差性が予測される生物種 |
| Full Name | F-box protein 7 |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 59-75 kDa |
| GenBank accession number | BC008361 |
| Gene Symbol | FBXO7 |
| Gene ID (NCBI) | 25793 |
| RRID | AB_3086324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3I1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBXO7, also named as FBX7, is a substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO7 recognizes BIRC2 and DLGAP5. It function in phosphorylation-dependent ubiquitination.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO7 antibody 30458-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |