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  • Featured Product
  • KD/KO Validated

FBXW2 Polyclonal antibody, PBS Only

FBXW2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11499-1-PBS

Synonyms

Md6, FWD2, FBXW 2, FBW2, F-box/WD repeat-containing protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using FBXW2 Polyclonal antibody (11499-1-AP)

    WB analysis of HEK-293 using 11499-1-AP (same clone as 11499-1-PBS)

    WB result of FBXW2 antibody (11499-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXW2 transfected HEK-293 cells. This data was developed using the same antibody clone with 11499-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FBXW2 Polyclonal antibody (11499-1-AP)

    WB analysis of HEK-293 using 11499-1-AP (same clone as 11499-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11499-1-AP (FBXW2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11499-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FBXW2 Polyclonal antibody (11499-1-AP)

    IHC staining of human colon cancer using 11499-1-AP (same clone as 11499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11499-1-AP (FBXW2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11499-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11499-1-PBS targets FBXW2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXW2 fusion protein Ag2045 相同性解析による交差性が予測される生物種
Full Name F-box and WD repeat domain containing 2
Calculated molecular weight 44 kDa, 52 kDa
Observed molecular weight 52 kDa, 43 kDa
GenBank accession numberBC018738
Gene symbol FBXW2
Gene ID (NCBI) 26190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The human FBW2 polypeptide contains an N-terminal F-box motif and a C-terminal domain of five WD repeats. A WD repeat is a motif typically consisting of 44-60 amino acids with a signature tryptophan and aspartic acid dipeptide at its C-terminus. The WD repeats in FBW2 recognize GCM1 in a way that is facilitated by GSK3b. Two isoforms were produced by alternative splicing with predicted MW of 52 and 43 kDa according to UniProt, and Catalog#11499-1-AP can recognise both.