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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXW4 Polyclonal antibody, PBS Only

FBXW4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10657-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using FBXW4 Polyclonal antibody (10657-1-AP)

    WB analysis of mouse lung using 10657-1-AP (same clone as 10657-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 10657-1-AP (FBXW4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10657-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using FBXW4 Polyclonal antibody (10657-1-AP)

    WB analysis of mouse liver using 10657-1-AP (same clone as 10657-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10657-1-AP (FBXW4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10657-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using FBXW4 Polyclonal antibody (10657-1-AP)

    IHC staining of human pancreas using 10657-1-AP (same clone as 10657-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10657-1-AP (FBXW4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10657-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using FBXW4 Polyclonal antibody (10657-1-AP)

    IHC staining of human pancreas using 10657-1-AP (same clone as 10657-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10657-1-AP (FBXW4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10657-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10657-1-PBS targets FBXW4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXW4 fusion protein Ag1047 相同性解析による交差性が予測される生物種
Full Name F-box and WD repeat domain containing 4
Calculated molecular weight 46 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC007380
Gene symbol FBXW4
Gene ID (NCBI) 6468
RRIDAB_2102754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. FBXW4, also known as SHFM3, encodes F-box and WD-40 domain-containing protein 4. Defects in SHFM3 are a cause of split-hand/foot malformation type 3 (SHFM3), an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits.