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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXW4 Polyclonal antibody, PBS Only

FBXW4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10657-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using FBXW4 Polyclonal antibody (10657-1-AP)

    WB analysis of mouse lung using 10657-1-AP (same clone as 10657-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 10657-1-AP (FBXW4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10657-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using FBXW4 Polyclonal antibody (10657-1-AP)

    WB analysis of mouse liver using 10657-1-AP (same clone as 10657-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10657-1-AP (FBXW4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10657-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using FBXW4 Polyclonal antibody (10657-1-AP)

    IHC staining of human pancreas using 10657-1-AP (same clone as 10657-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10657-1-AP (FBXW4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10657-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using FBXW4 Polyclonal antibody (10657-1-AP)

    IHC staining of human pancreas using 10657-1-AP (same clone as 10657-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10657-1-AP (FBXW4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10657-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10657-1-PBS targets FBXW4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXW4 fusion protein Ag1047 相同性解析による交差性が予測される生物種
Full Name F-box and WD repeat domain containing 4
Calculated molecular weight 46 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC007380
Gene symbol FBXW4
Gene ID (NCBI) 6468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. FBXW4, also known as SHFM3, encodes F-box and WD-40 domain-containing protein 4. Defects in SHFM3 are a cause of split-hand/foot malformation type 3 (SHFM3), an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits.