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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXW5 Polyclonal antibody, PBS Only

FBXW5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18321-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FBXW5 Polyclonal antibody (18321-1-AP)

    WB analysis using 18321-1-AP (same clone as 18321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18321-1-AP (FBXW5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FBXW5 Polyclonal antibody (18321-1-AP)

    IHC staining of mouse testis using 18321-1-AP (same clone as 18321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FBXW5 Polyclonal antibody (18321-1-AP)

    IHC staining of mouse testis using 18321-1-AP (same clone as 18321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FBXW5 Polyclonal antibody (18321-1-AP)

    IHC staining of human kidney using 18321-1-AP (same clone as 18321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FBXW5 Polyclonal antibody (18321-1-AP)

    IHC staining of human kidney using 18321-1-AP (same clone as 18321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18321-1-PBS targets FBXW5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXW5 fusion protein Ag13163 相同性解析による交差性が予測される生物種
Full Name F-box and WD repeat domain containing 5
Calculated molecular weight 64 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC014297
Gene symbol FBXW5
Gene ID (NCBI) 54461
RRIDAB_2878530
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. FBXW5 (F-box/WD repeat-containing protein 5) is a novel protein containing WD-40 repeats, which were frequently involved in protein-protein interactions. FBXW5 negatively regulates TAK1 in the IL-1beta signaling pathway. By forming different E3 ubiquitin-protein ligase complexes, FBXW5 mediates the polyubiquitination and subsequent degradation of TSC2 and centriolar protein HsSAS-6.