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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXW5 Polyclonal antibody, PBS Only

FBXW5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18321-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXW5 Polyclonal antibody (18321-1-AP)

    WB analysis using 18321-1-AP (same clone as 18321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18321-1-AP (FBXW5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FBXW5 Polyclonal antibody (18321-1-AP)

    IHC staining of mouse testis using 18321-1-AP (same clone as 18321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FBXW5 Polyclonal antibody (18321-1-AP)

    IHC staining of mouse testis using 18321-1-AP (same clone as 18321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FBXW5 Polyclonal antibody (18321-1-AP)

    IHC staining of human kidney using 18321-1-AP (same clone as 18321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FBXW5 Polyclonal antibody (18321-1-AP)

    IHC staining of human kidney using 18321-1-AP (same clone as 18321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18321-1-PBS targets FBXW5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXW5 fusion protein Ag13163 相同性解析による交差性が予測される生物種
Full Name F-box and WD repeat domain containing 5
Calculated molecular weight 64 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC014297
Gene symbol FBXW5
Gene ID (NCBI) 54461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. FBXW5 (F-box/WD repeat-containing protein 5) is a novel protein containing WD-40 repeats, which were frequently involved in protein-protein interactions. FBXW5 negatively regulates TAK1 in the IL-1beta signaling pathway. By forming different E3 ubiquitin-protein ligase complexes, FBXW5 mediates the polyubiquitination and subsequent degradation of TSC2 and centriolar protein HsSAS-6.