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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using mFC tag Monoclonal antibody (67122-1-Ig)

    WB analysis using 67122-1-Ig

    67122-1-Ig was lysised and subjected to SDS-PAGE followed by western blot with 67122-1-Ig and isotype control antibody 66360-1-Ig at dilution of 1:5000 incubated at room temperature for 1.5 hours. HRP-goat anti-mouse kappa chain secondary antibody was used for detection. .

  • Western Blot (WB) analysis of various lysates using mFC tag Monoclonal antibody (67122-1-Ig)

    WB analysis using 67122-1-Ig

    0.25 μL mouse serum was subjected to SDS-PAGE followed by western blot with 67122-1-Ig and isotype control antibody 66360-1-Ig at dilution of 1:10000 incubated at room temperature for 1.5 hours. HRP-goat anti-mouse kappa chain secondary antibody was used for detection. .

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mFC tag Monoclonal antibody

mFC tag Monoclonal Antibody for WB, ELISA
Cat No. 67122-1-Ig
Clone No.2F5A2

Host / Isotype

Mouse / IgG1

Reactivity

mouse

Applications

WB, ELISA

Synonyms

FC tag, mFC tag

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMouse IgG

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67122-1-Ig targets mFC tag in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen mFC tag fusion protein Ag27844 相同性解析による交差性が予測される生物種
Full Name mFC tag
Calculated molecular weight 26 kDa
Observed molecular weight25-30 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918482
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the mouse Fc tag. It's mouse Fc gamma 2a.

Protocols

Product Specific Protocols
WB protocol for mFC tag antibody 67122-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols