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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FCER1G Polyclonal antibody (13566-1-AP)

    WB analysis using 13566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13566-1-AP (FCER1G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FCER1G Polyclonal antibody (13566-1-AP)

    IHC staining of human ovary cancer using 13566-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13566-1-AP (FCER1G antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using FCER1G Polyclonal antibody (13566-1-AP)

    IF Staining of THP-1 using 13566-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCER1G antibody (13566-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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FCER1G Polyclonal antibody

FCER1G Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13566-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FcRgamma, FCRG, FceRI gamma, Fc-epsilon RI-gamma, Fc receptor gamma-chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13566-1-AP targets FCER1G in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FCER1G fusion protein Ag4442 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
Calculated molecular weight 87 aa, 10 kDa
Observed molecular weight10 kDa
GenBank accession numberBC033872
Gene Symbol FcRγ
Gene ID (NCBI) 2207
RRIDAB_3669165
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FCER1G (Fc receptor gamma-chain, FcRgamma), also known as Fc-epsilon RI-gamma, or IgE Fc receptor subunit gamma (FceRI gamma), is a component of the high-affinity immunoglobulin E (IgE) receptor (Fc epsilon RI) which is a tetrameric complex of one alpha subunit, one beta subunit, and two disulfide-linked gamma subunits (PMID: 2146219; 2531187). Fc epsilon RI is present exclusively on mast cells and basophils, mediating allergic inflammatory signaling (PMID: 17438574). FCER1G is widely expressed and contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors (PMID: 19098920).

Protocols

Product Specific Protocols
WB protocol for FCER1G antibody 13566-1-APDownload protocol
IHC protocol for FCER1G antibody 13566-1-APDownload protocol
IF protocol for FCER1G antibody 13566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols