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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FcRγ Polyclonal antibody

FcRγ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13566-1-AP

Synonyms

FCER1G, FCRG, FceRI gamma, Fc-epsilon RI-gamma, Fc receptor gamma-chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FcRγ Polyclonal antibody (13566-1-AP)

    WB analysis using 13566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13566-1-AP (FcRγ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FcRγ Polyclonal antibody (13566-1-AP)

    IHC staining of human ovary cancer using 13566-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13566-1-AP (FcRγ antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using FcRγ Polyclonal antibody (13566-1-AP)

    IF Staining of THP-1 using 13566-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FcRγ antibody (13566-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inRAW 264.7 cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13566-1-AP targets FcRγ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FcRγ fusion protein Ag4442 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
Calculated molecular weight 87 aa, 10 kDa
Observed molecular weight10 kDa
GenBank accession numberBC033872
Gene symbol FcRγ
Gene ID (NCBI) 2207
RRIDAB_3669165
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FcRγ (Fc receptor gamma-chain, FcRgamma), also known as Fc-epsilon RI-gamma, or IgE Fc receptor subunit gamma (FceRI gamma), is a component of the high-affinity immunoglobulin E (IgE) receptor (Fc epsilon RI) which is a tetrameric complex of one alpha subunit, one beta subunit, and two disulfide-linked gamma subunits (PMID: 2146219; 2531187). Fc epsilon RI is present exclusively on mast cells and basophils, mediating allergic inflammatory signaling (PMID: 17438574). FcRγ is widely expressed and contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors (PMID: 19098920).

Protocols

Product Specific Protocols
WB protocol for FcRγ antibody 13566-1-APDownload protocol
IHC protocol for FcRγ antibody 13566-1-APDownload protocol
IF protocol for FcRγ antibody 13566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols