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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD64 Polyclonal antibody

CD64 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27563-1-AP

Synonyms

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, IGFR1, IgG Fc receptor I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD64 Polyclonal antibody (27563-1-AP)

    WB analysis using 27563-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CD64 Polyclonal antibody (27563-1-AP)

    WB analysis of HL-60 using 27563-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD64 Polyclonal antibody (27563-1-AP)

    WB analysis of U-937 using 27563-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CD64 Polyclonal antibody (27563-1-AP)

    IF Staining of THP-1 using 27563-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD64 antibody (27563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inTHP-1 cells, U-937 cells, HL-60 cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27563-1-AP targets CD64 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD64 fusion protein Ag26727 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Calculated molecular weight 374 aa, 43 kDa
Observed molecular weight 65-70 kDa, 43-45 kDa
GenBank accession numberBC032634
Gene symbol FCGR1A
Gene ID (NCBI) 2209
RRIDAB_2880909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.

Protocols

Product Specific Protocols
WB protocol for CD64 antibody 27563-1-APDownload protocol
IF protocol for CD64 antibody 27563-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Cancer Cell-Derived Exosomes Promote HCC Tumorigenesis Through Hedgehog Pathway.

Authors - Li Li
View Article