• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FCGR2A / CD32a Polyclonal antibody

FCGR2A / CD32a Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27138-1-AP

Synonyms

CD32, Fc-gamma RII-a, FCG2, Fc gamma RIIa, Fc gamma RII a

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FCGR2A / CD32a Polyclonal antibody (27138-1-AP)

    WB analysis using 27138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27138-1-AP (FCGR2A / CD32a antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FCGR2A / CD32a Polyclonal antibody (27138-1-AP)

    IHC staining of human tonsillitis using 27138-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27138-1-AP (FCGR2A / CD32a antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FCGR2A / CD32a Polyclonal antibody (27138-1-AP)

    IHC staining of human tonsillitis using 27138-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27138-1-AP (FCGR2A / CD32a antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inU-937 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27138-1-AP targets FCGR2A / CD32a in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FCGR2A / CD32a fusion protein Ag25878 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Calculated molecular weight 35 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC020823
Gene symbol CD32a
Gene ID (NCBI) 2212
RRIDAB_2880770
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

Protocols

Product Specific Protocols
WB protocol for FCGR2A / CD32a antibody 27138-1-APDownload protocol
IHC protocol for FCGR2A / CD32a antibody 27138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols