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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FCGR2B / CD32b Polyclonal antibody

FCGR2B / CD32b Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21541-1-AP

Synonyms

FCGR2B, Fc-gamma RII-b, FCG2, Fc gamma RIIb, Fc gamma RII b

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using FCGR2B / CD32b Polyclonal antibody (21541-1-AP)

    WB analysis of human placenta using 21541-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using FCGR2B / CD32b Polyclonal antibody (21541-1-AP)

    WB analysis of Raji using 21541-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FCGR2B / CD32b Polyclonal antibody (21541-1-AP)

    IHC staining of human tonsillitis using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FCGR2B / CD32b Polyclonal antibody (21541-1-AP)

    IHC staining of human tonsillitis using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using FCGR2B / CD32b Polyclonal antibody (21541-1-AP)

    IHC staining of human placenta using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using FCGR2B / CD32b Polyclonal antibody (21541-1-AP)

    IHC staining of human placenta using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using FCGR2B / CD32b Polyclonal antibody (21541-1-AP)

    IHC staining of human spleen using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using FCGR2B / CD32b Polyclonal antibody (21541-1-AP)

    IHC staining of human spleen using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FCGR2B / CD32b Polyclonal antibody (21541-1-AP)

    IHC staining of human tonsillitis using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman placenta tissue, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21541-1-AP targets FCGR2B / CD32b in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FCGR2B / CD32b fusion protein Ag16189 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIb, receptor (CD32)
Calculated molecular weight 310 aa, 34 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC031992
Gene symbol CD32b
Gene ID (NCBI) 2213
RRIDAB_2878881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FCGR2B / CD32b antibody 21541-1-APDownload protocol
IHC protocol for FCGR2B / CD32b antibody 21541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Ther

High-dose systemic adeno-associated virus vector administration causes liver and sinusoidal endothelial cell injury

Authors - Juliette Hordeaux
View Article